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Nat Prod Res. 2019 Mar 19:1-5. doi: 10.1080/14786419.2019.1582041. [Epub ahead of print]

Metabolite profiling of Huaiyang Medicago polymorpha with different mowing crops.

Author information

1
a State Key Laboratory of Dao-di Herbs (preparing) , Experimental Research Center, China Academy of Chinese Medical Sciences , Beijing , China.
2
b Zhong Yuan Academy of Biological Medicine , Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University , Liaocheng , China.
3
c Health Preservation Institute, Beijing University of Chinese Medicine , Beijing , China.
4
d Pharmacy School , Inner Mongolian Medical University , Hohhot , China.

Abstract

The main purpose of our study was to identify and compare secondary metabolites due to different mowing in order to make better use of Huaiyang Medicago polymorpha. The metabolite profiling of Huaiyang Medicago polymorpha with two mowing crops was performed using a rapid resolution liquid chromatography system with quadrupole time-of-flight mass spectrometer (RRLC-QTOFMS) followed by multivariate statistical analyses. Principal Component Analysis (PCA) results showed a clear distinction between two mowing crops. The major metabolites that contributed to mowing discrimination were identified. The results also showed that the content of major active compounds in Medicago polymorpha from the second crop are higher significantly than the first crop. This study suggests that the strategy is a reliable and simple method for the rapid discrimination of subtle variations due to different mowing crops.

KEYWORDS:

Huaiyang ; RRLC-QTOFMS; metabolite profiling; multivariate statistical analysis

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