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Cell Tissue Bank. 2019 Jun;20(2):173-182. doi: 10.1007/s10561-019-09751-0. Epub 2019 Mar 18.

A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria.

Author information

1
School of Medicine and Life Science, Shandong Academy of Medical Sciences, University of Jinan, 18877, Jingshi Road, Jinan, 250012, Shandong, China.
2
Key Laboratory for Rare Disease Research of Shandong Province, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, 18877, Jingshi Road, Jinan, 250012, Shandong, China.
3
Department of Neurosurgery, Shandong Cancer Hospital affiliated to Shandong University, 440 Jiyan Road, Jinan, 250114, Shandong, China.
4
Key Laboratory for Rare Disease Research of Shandong Province, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, 18877, Jingshi Road, Jinan, 250012, Shandong, China. doctorwy@163.com.

Abstract

Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests.

KEYWORDS:

Calvaria; Comparative; Mouse; Osteoblast; Rat

PMID:
30887273
DOI:
10.1007/s10561-019-09751-0

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