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Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6. Epub 2019 Mar 18.

A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis.

Author information

1
Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
2
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA.
3
School of Life Sciences, Westlake Institute for Advanced Study, Westlake University, Hangzhou, China.
4
Department of Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA, USA.
5
Donald Danforth Plant Science Center, St. Louis, MO, USA.
6
Department of Biology, Stanford University, Stanford, CA, USA.
7
Salk Institute for Biological Studies, La Jolla, CA, USA.
8
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
9
Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN, USA.
10
Department of Molecular Biology, Princeton University, Princeton, NJ, USA. mjonikas@princeton.edu.
11
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA. mjonikas@princeton.edu.

Abstract

Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized1,2. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii. The 62,389 mutants in the library, covering 83% of nuclear protein-coding genes, are available to the community. Each mutant contains unique DNA barcodes, allowing the collection to be screened as a pool. We performed a genome-wide survey of genes required for photosynthesis, which identified 303 candidate genes. Characterization of one of these genes, the conserved predicted phosphatase-encoding gene CPL3, showed that it is important for accumulation of multiple photosynthetic protein complexes. Notably, 21 of the 43 higher-confidence genes are novel, opening new opportunities for advances in understanding of this biogeochemically fundamental process. This library will accelerate the characterization of thousands of genes in algae, plants, and animals.

PMID:
30886426
PMCID:
PMC6636631
DOI:
10.1038/s41588-019-0370-6
[Indexed for MEDLINE]
Free PMC Article

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