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Enzyme Microb Technol. 2019 Jun;125:45-52. doi: 10.1016/j.enzmictec.2019.02.010. Epub 2019 Feb 28.

Strategy and regulatory mechanisms of glutamate feeding to enhance astaxanthin yield in Xanthophyllomyces dendrorhous.

Author information

1
College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, PR China; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou 362000, PR China.
2
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
3
SDIC Biotechnology Investment Co. Ltd, State Development and Investment Corporation, Beijing 100034, PR China.
4
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China. Electronic address: xpling@xmu.edu.cn.
5
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China. Electronic address: ylu@xmu.edu.cn.

Abstract

Xanthophyllomyces dendrorhous is an excellent industrial source for production of natural astaxanthin, but the yield of astaxanthin is relative low due to the contradiction between biomass weight and astaxanthin accumulation. Glutamate, a metabolite connecting nitrogen and carbon metabolisms, is probably a promising entry point to interfere cellular metabolisms. Thus, the effect of glutamate on cell growth and astaxanthin accumulation in X. dendrorhous was investigated. Results showed that glutamate feeding facilitated glucose consumption and further led to the increment of astaxanthin content with little influence of cell growth. A comparative proteomics study was applied to decipher the regulatory mechanisms of enhanced astaxanthin biosynthesis in X. dendrorhous as a response to the glutamate feeding. The expressions of proteins with the highest degree of fold change were involved in carbohydrate, amino acids, and carotenogenesis metabolisms as well as redox and stress-associated metabolisms. In addition, a possible regulatory model of enhanced astaxanthin accumulation in response to glutamate feeding in X. dendrorhous is also proposed.

KEYWORDS:

Astaxanthin; Glutamate; Proteomics; Xanthophyllomyces dendrorhous

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