Format

Send to

Choose Destination
Eur J Immunol. 2019 Mar 18. doi: 10.1002/eji.201847897. [Epub ahead of print]

Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans.

Author information

1
Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
2
Renal Transplant Unit, Division of Internal Medicine, Academic Medical Centre, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
3
Sanquin Research and Landsteiner laboratory, Amsterdam, The Netherlands.
4
Institute for Immunology, University Medical Centre Freiburg, Freiburg, Germany.

Abstract

During acute viral infections in mice, IL-7Rα and KLRG1 together are used to distinguish the short-lived effector cells (SLEC; IL-7Rαlo KLRGhi ) from the precursors of persisting memory cells (MPEC; IL-7Rαhi KLRG1lo ). We here show that these markers can be used to define distinct subsets in the circulation and lymph nodes during the acute phase and in "steady state" in humans. In contrast to the T cells in the circulation, T cells derived from lymph nodes hardly contain any KLRG1-expressing cells. The four populations defined by IL-7Rα and KLRG1 differ markedly in transcription factor, granzyme and chemokine receptor expression. When studying renal transplant recipients experiencing a primary hCMV and EBV infection, we also found that after viral control, during latency, Ki-67-negative SLEC can be found in the peripheral blood in considerable numbers. Thus, combined analyses of IL-7Rα and KLRG1 expression on human herpes virus-specific CD8+ T cells can be used to separate functionally distinct subsets in humans. As a noncycling IL-7Rαlo KLRG1hi population is abundant in healthy humans, we conclude that this combination of markers not only defines short-lived effector cells during the acute response but also stable effector cells that are formed and remain present during latent herpes infections.

KEYWORDS:

Human T-cell memory; IL-7 receptor-α-chain; Killer cell lectin-like receptor subfamily G member 1; Memory precursor effector cells; Short-lived effector cells; Viral infection

PMID:
30883723
DOI:
10.1002/eji.201847897

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center