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Epigenomics. 2019 Mar 18. doi: 10.2217/epi-2019-0002. [Epub ahead of print]

Genome-wide screening of altered m6A-tagged transcript profiles in the hippocampus after traumatic brain injury in mice.

Author information

1
CNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences & Genomics, State Key Laboratory of Medical Genomics, Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, PR China.
2
Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, PR China.
3
Department of Anesthesiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, Pudong New District, Shanghai 200127, PR China.
4
Department of Neurosurgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, Guangdong Province, PR China.

Abstract

AIM:

To systematically profile RNA m6A modification landscape after traumatic brain injury (TBI) in mice.

MATERIALS & METHODS:

Expression of m6A-related genes was detected by quantitative real-time PCR (qPCR). Expression and location of METTL3, a key component of m6A methyltransferase complex, were determined by immunostaining. Genome-wide profiling of m6A-tagged transcripts was conducted by m6A-modified RNA immunoprecipitation sequencing (m6A-RIP-seq) and RNA sequencing (RNA-seq).

RESULTS:

METTL3 was downregulated after TBI. In total, 922 m6A peaks were differentially expressed as determined by m6A-RIP-seq, with 370 upregulated and 552 downregulated. In addition, we identified differentially expressed hypomethylated and hypermethylated mRNA transcripts.

CONCLUSION:

Our data provided novel information regarding m6A modification changes in the early period of TBI, which might be promising therapy targets.

KEYWORDS:

N6-methyladenosine (m6A); hippocampus; traumatic brain injury (TBI)

PMID:
30882247
DOI:
10.2217/epi-2019-0002

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