Format

Send to

Choose Destination
Life Sci. 2019 Apr 15;223:110-119. doi: 10.1016/j.lfs.2019.03.025. Epub 2019 Mar 14.

Cordycepin suppresses cell proliferation and migration by targeting CLEC2 in human gastric cancer cells via Akt signaling pathway.

Author information

1
Cancer Research Center, Yanbian University, Jilin, Yanbian 133002, China; Key Laboratory of the Science and Technology Department of Jilin Province, China; Key Laboratory of the Changbai Mountain, Yanbian University, Jilin, Yanbian 133002, China.
2
Cancer Research Center, Yanbian University, Jilin, Yanbian 133002, China; Key Laboratory of the Science and Technology Department of Jilin Province, China; Key Laboratory of the Changbai Mountain, Yanbian University, Jilin, Yanbian 133002, China. Electronic address: renxsh@ybu.edu.cn.

Abstract

PURPOSE:

Gastric cancer is a common malignancy worldwide, and is associated with high morbidity and mortality rates. Cordycepin is a 3'-deoxyadenosine drug with significant anti-cancer effects. The aim of this study was to determine the molecular mechanisms underlying cordycepin action on gastric cancer cell proliferation and migration.

METHODS:

The human gastric cancer cell lines MGC-803 and HGC-27 were treated with different concentrations of cordycepin (25 μM, 50 μM, 100 μM and 5 μM, 25 μM and 50 μM) for 48 h. Cell proliferation was assessed by MTT and colony formation assays, and in vitro migration by the wound healing and transwell assays. In addition, Flow Cytometry was used to detect the cell cycle and apoptosis. RT-PCR and Western blotting were used to evaluate the expression levels of key factors.

RESULTS:

Cordycepin significantly inhibited gastric cancer cell proliferation and migration in a dose-dependent manner, in addition to inducing apoptosis and arresting the cell cycle at the G2 phase. Mechanistically, cordycepin targeted the PI3K/Akt signaling pathway by significantly altering the expression levels/activation of several key mediators, and upregulated the anti-metastatic factor CLEC2.

CONCLUSION:

Cordycepin inhibited the proliferation and migration of gastric cancer cells by upregulating CLEC2 via the Akt signaling pathway.

KEYWORDS:

Apoptosis; CLEC2; Cell cycle; Cordycepin; EMT; Gastric cancer

PMID:
30878262
DOI:
10.1016/j.lfs.2019.03.025
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center