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Ecotoxicology. 2019 Mar 14. doi: 10.1007/s10646-019-02030-4. [Epub ahead of print]

Chronic toxicity and biochemical response of Apis cerana cerana (Hymenoptera: Apidae) exposed to acetamiprid and propiconazole alone or combined.

Han W1,2, Yang Y1,2, Gao J3,4, Zhao D5,6, Ren C7, Wang S1,2, Zhao S1,2, Zhong Y1,2.

Author information

1
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
2
Bee Industry Technology Research Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
3
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. jinglin.g@163.com.
4
Bee Industry Technology Research Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. jinglin.g@163.com.
5
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. dongxiangzh@163.com.
6
Bee Industry Technology Research Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. dongxiangzh@163.com.
7
Hainan Bosswell Agrichemical Co., Ltd, Haikou, China.

Abstract

Acetamiprid and ergosterol-inhibiting fungicide (EBI) are frequently applied to many flowering plants, while honey bees are pollinating agents or pollinators of the flowers. Hence honey bees are often exposed to these pesticides. But until now, the effects of theses combinations at field-realistic doses on honey bee health have been poorly investigated. In this study, we explore the synergistic mortality and some physiological effects in surviving honey bees after chronic oral exposure to acetamiprid and/or propiconazole in the laboratory. The results indicated that chronic combined exposure to acetamiprid and propiconazole produced a significant synergistic effect on mortality both for newly emerged bees (50% mortality in 7.2 days) and forager bees (50% mortality in 4.8 days). Honey bee weight of newly emerged bees was decreased after feeding food with a field concentration of acetamiprid and propiconazole, alone or combined for 10 days. Combination of acetamiprid and propiconazole also modulated the activities of P450s, GST and CAT in newly emerged bees and forager bees than either alone, but neither pesticide affected the activity of AChE. These results show that chronic combined exposure to pesticides of relatively low toxicity may caused severely physiological disruptions that could be potentially damaging for the honey bees.

KEYWORDS:

Apis cerana cerana; Detoxification enzymes; Physiological effects; Synergistic mortality

PMID:
30874992
DOI:
10.1007/s10646-019-02030-4

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