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J Craniofac Surg. 2019 May/Jun;30(3):777-783. doi: 10.1097/SCS.0000000000005392.

Adipogenic Mesenchymal Stem Cells and Hyaluronic Acid as a Cellular Compound for Bone Tissue Engineering.

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Department of Dental Prosthesis, Pontifical Catholic University of Rio Grande do Sul - PUCRS.
Experimental Cardiology Center, Institute of Cardiology.
Institute of Petroleum and Natural Resources - PUCRS.
Department of Biochemistry, Federal University of Rio Grande do Sul - UFRGS.
Laboratory of Cell Biology and Respiratory Diseases.
Department of Oral Surgery, Pontifical Catholic University of Rio Grande do Sul - PUCRS, Porto Alegre, Brazil.


This study investigates the applicability of adipose mesenchymal stem cells (mADSCs) and hyaluronic acid (HA) as a cellular compound for bone tissue engineering. A critical bone defect was created on each femur of 25 rats in vivo, receiving the following 5 graft treatments: I-Control-defect; II-HA; III-mADSCs; IV-mADSCs+HA; and V-previously osteoinduced mADSCs+HA. Evaluation using microcomputed tomography, histomorphometry, and RT-PCR analysis was performed 23 days after implantation. Microcomputed tomography analysis indicated higher means of bone contact surface (BCS) and bone surface density (BSD) for the mADSCs+HA group compared with Control and the HA groups (P < 0.05). Histomorphometric findings showed higher means of bone regeneration in the mADSCs+HA compared with HA and Control groups (P < 0.05). The RT-PCR ratios showed no difference in type 1 collagen (Col1A) gene expression or osteopontin (OP) gene expression, whereas for the osteonectin gene (ON) higher means were found in the HA and mADSCs osteoin+HA groups (P < 0.05). These results suggest that a combination of HA and mADSCs without prior osteoinduction might be applicable for bone tissue regeneration.

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