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3 Biotech. 2019 Apr;9(4):123. doi: 10.1007/s13205-019-1663-2. Epub 2019 Mar 5.

Characterization, solubility and antibacterial activity of inclusion complex of questin with hydroxypropyl-β-cyclodextrin.

Guo L1,2,3, Cao X1,2, Yang S1,2, Wang X1,2, Wen Y2, Zhang F1,2, Chen H1,2, Wang L1,2.

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1Jiangsu Key Laboratory of Marine Bioresources and Environment, Huaihai Institute of Technology, Lianyungang, 222005 China.
2Jiangsu Key Laboratory of Marine Biotechnology, Co-innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005 China.
Jiangsu Institute of Marine Resources Development, Lianyungang, 222004 China.


To increase the water solubility of questin and broaden its application in preventing and treating Vibrio diseases in aquaculture, an inclusion complex of questin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared by stirring and coevaporation strategy. The results of thin-layer chromatography and nuclear magnetic resonance spectrum confirmed the inclusion of questin into HP-β-CD. The aqueous solubility of questin in the inclusion complex reached (62.63 ± 1.21) µg/mL, which was 110 times of questin's original solubility. The preliminary agar diffusion method indicated that questin-HP-β-CD inclusion complex showed enhanced antibacterial activity against Vibrio harveyi compared with free questin. This finding provided a reliable basis for the further application of questin as aquatic antibacterial agent.


2-Hydroxypropyl-β-cyclodextrin; Antibacterial activity; Inclusion complex; Questin; Solubility

[Available on 2020-04-01]

Conflict of interest statement

The authors declare that they have no conflict of interest.This article does not contain any studies with human participants or animals performed by any of the authors.

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