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Microb Pathog. 2019 May;130:178-185. doi: 10.1016/j.micpath.2019.03.007. Epub 2019 Mar 9.

Characterisation and anti-biofilm activity of glycerol monolaurate nanocapsules against Pseudomonas aeruginosa.

Author information

1
Post Graduate Program in Nanosciences, Universidade Franciscana, Santa Maria, Brazil; Microbiology and Parasitology Department, Health Sciences Center, Universidade Federal de Santa Maria, Santa Maria, Brazil. Electronic address: leonardoquintanalopes@gmail.com.
2
Laboratory of Research in Biochemistry and Molecular Biology of Microorganisms, Post Graduate Program in Biochemistry and Bioprospecting, Universidade Federal de Pelotas, Capão Do Leão, Brazil.
3
Pharmacy Department, Faculdade Anhanguera, Pelotas, Brazil.
4
Universidade Federal do Pampa, Bage, Brazil.
5
Post Graduate Program in Nanosciences, Universidade Franciscana, Santa Maria, Brazil; Microbiology and Parasitology Department, Health Sciences Center, Universidade Federal de Santa Maria, Santa Maria, Brazil.

Abstract

Pseudomonas aeruginosa is a ubiquitous microorganism that commonly causes hospital-acquired infections, including pneumonia, bloodstream and urinary tract infections and it is well known for chronically colonising the respiratory tract of patients with cystic fibrosis, causing severe intermittent exacerbation of the condition. P. aeruginosa may appear in the free form cell but also grows in biofilm communities adhered to a surface. An alternative to conventional antimicrobial agents are nanoparticles that can act as carriers for antibiotics and other drugs. In this context, the study aimed to characterise and verify the anti-biofilm potential of GML Nanocapsules against P. aeruginosa. The nanocapsules showed a mean diameter of 190.7 nm, polydispersion index of 0.069, the zeta potential of -23.3 mV. The microdilution test showed a MIC of 62.5 μg/mL to GML and 15.62 μg/mL to GML Nanocapsules. The anti-biofilm experiments demonstrated the significant reduction of biomass, proteins, polysaccharide and viable P. aeruginosa in biofilm treated with GML Nanocapsules while the free GML did not cause an effect. The AFM images showed a decrease in a biofilm which received GML. The positive results suggest an alternative for the public health trouble related to infections associated with biofilm.

KEYWORDS:

Atomic force microscopy; Biofilm; Glycerol monolaurate; Nanocapsules; P. aeruginosa

PMID:
30862561
DOI:
10.1016/j.micpath.2019.03.007
[Indexed for MEDLINE]

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