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Prep Biochem Biotechnol. 2019 Mar 12:1-9. doi: 10.1080/10826068.2018.1541806. [Epub ahead of print]

Comparative proteomic analysis revealed the metabolic mechanism of excessive exopolysaccharide synthesis by Bacillus mucilaginosus under CaCO3 addition.

Xu H1,2, Zhang Z2, Li H2, Yan Y2, Shi J2, Xu Z1,2,3.

Author information

1
a National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology , Jiangnan University , Wuxi , P. R. China.
2
b Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences , Jiangnan University , Wuxi , P. R. China.
3
c Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology , Jiangnan University , Wuxi , P. R. China.

Abstract

The metabolic mechanism of excessive exopolysaccharide (BMPS) synthesis by Bacillus mucilaginosus CGMCC5766 under CaCO3 addition was investigated. Under CaCO3 (5 g/L), the maximum BMPS concentration reached 28.4 g/L, which was 11.2 folds higher than that of the control. Proteomics was then used to analyze the proteins with substantial differences expressed by B. mucilaginosus with and without CaCO3 addition. The proteomic results revealed that the enzymes related to the central metabolic pathway, amino acid biosynthesis, and nucleotide metabolism were depressed. By contrast, the UDP-glucose pyrophosphorylase involved in BMPS biosynthesis was overexpressed and converted metabolic flux from the biomass accumulation to the biosynthesis of BMPS. This research provides a new and widened perspective into understanding the mechanism of BMPS biosynthesis and applying theoretical and practical significance for the improvement of BMPS production from B. mucilaginosus.

KEYWORDS:

; CaCO; exopolysaccharide; proteomic

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