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Int J Mycobacteriol. 2019 Jan-Mar;8(1):70-74. doi: 10.4103/ijmy.ijmy_9_19.

Upregulated bovine tuberculosis microRNAs Trigger oncogenic pathways: An In silico perception.

Author information

1
Department of Animal Production and Fisheries, Laboratory of Animal Physiology and Health, Institute of Agricultural Research for Development, Bambui, Cameroon.
2
Department of Computational Biology and Bioinformatics, University of Kerala, Thiruvananthapuram, Kerala, India.
3
Division of Immunology and South Africa Medical Research Council, Immunology of Infectious Disease, Faculty of Health Sciences, University of Cape Town, Institute of Infectious Diseases and Molecular Medicine, South Africa; Department of Biochemistry, University of Douala, Cameroon.
4
System Biology Laboratory, Chantal Biya International Reference Center, Yaounde; Department of Biology, Higher Teachers' Training College, University of Yaounde I, Cameroon.

Abstract

Background/Objective:

Although microRNA (miRNA)-directed regulation of bovine tuberculosis (bTB) has already been reported, very little is known about the incited pathways and genes. We profiled bTB-upregulated miRNAs through an in silico methodology.

Methods:

The data of upregulated miRNAs in bTB versus healthy controls were collected and clustered into three groups by their tissue specificity as follows: G1 (mammary gland-specific): bta-miR-146a; G2 (peripheral blood mononuclear cell-specific): bta-miR-155; and G3 (alveolar macrophage-specific): bta-miR-146a, bta-miR-155, bta-miR-142-5p, bta-miR-423-3p, bta-miR-21-5p, bta-miR-27a-3p, bta-miR-99b, bta-miR-147, bta-miR-223, and bta-let-7i. The miRNA-mRNA interaction network was defined by TargetScan. The gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways of these transcripts were examined.

Results:

The results illustrate the induction of pathways in cancer, highly enriched, and unanimous to all three gene sets (G1, G2, and G3). Mitogen-activated protein kinase and PI3K-Akt signaling were specific to G2 and G3 with fibroblast growth factors formed the key factors.

Conclusion:

The inferred cancer cascades denote a probable modulation of innate immune response in an infectious state. These baseline pictures could lay the ground for further substantive studies.

KEYWORDS:

Bovine tuberculosis; computational; microRNA; pathway prediction; target gene

PMID:
30860182
DOI:
10.4103/ijmy.ijmy_9_19
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