Format

Send to

Choose Destination
Genome Res. 2019 Mar 11. doi: 10.1101/gr.239442.118. [Epub ahead of print]

Coexpression patterns define epigenetic regulators associated with neurological dysfunction.

Author information

1
Human Genetics Training Program, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
2
McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
3
Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112, USA.
4
Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia.
5
Department of Medical Biology, The University of Melbourne, Parkville, Victoria 3010, Australia.
6
Department of Biomedical Informatics, University of Utah, Salt Lake City, Utah 84108, USA.
7
USTAR Center for Genetic Discovery, University of Utah, Salt Lake City, Utah 84108, USA.
8
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
9
Faculty of Medicine, University of Iceland, 101 Reykjavík, Iceland.
10
Landspitali University Hospital, 101 Reykjavík, Iceland.
11
Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.

Abstract

Coding variants in epigenetic regulators are emerging as causes of neurological dysfunction and cancer. However, a comprehensive effort to identify disease candidates within the human epigenetic machinery (EM) has not been performed; it is unclear whether features exist that distinguish between variation-intolerant and variation-tolerant EM genes, and between EM genes associated with neurological dysfunction versus cancer. Here, we rigorously define 295 genes with a direct role in epigenetic regulation (writers, erasers, remodelers, readers). Systematic exploration of these genes reveals that although individual enzymatic functions are always mutually exclusive, readers often also exhibit enzymatic activity (dual-function EM genes). We find that the majority of EM genes are very intolerant to loss-of-function variation, even when compared to the dosage sensitive transcription factors, and we identify 102 novel EM disease candidates. We show that this variation intolerance is driven by the protein domains encoding the epigenetic function, suggesting that disease is caused by a perturbed chromatin state. We then describe a large subset of EM genes that are coexpressed within multiple tissues. This subset is almost exclusively populated by extremely variation-intolerant genes and shows enrichment for dual-function EM genes. It is also highly enriched for genes associated with neurological dysfunction, even when accounting for dosage sensitivity, but not for cancer-associated EM genes. Finally, we show that regulatory regions near epigenetic regulators are genetically important for common neurological traits. These findings prioritize novel disease candidate EM genes and suggest that this coexpression plays a functional role in normal neurological homeostasis.

PMID:
30858344
DOI:
10.1101/gr.239442.118
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center