Abstract
A procedure for examining possible sequence homology in the triplet neurofilament proteins using a sodium dodecyl sulfate-polyacrylamide gel electrophoresis system is described. Five different chemical reagents (cyanogen bromide, BNPS-skatole, hydroxylamine, formic acid, and nitrothiocyanobenzoic acid) have been used for peptide mapping studies. Potential applications of this technique are discussed.
MeSH terms
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Amino Acid Sequence
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Animals
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Cattle
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Cyanogen Bromide
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Electrophoresis, Polyacrylamide Gel / methods*
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Formates
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Hydroxylamine
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Hydroxylamines
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Intermediate Filament Proteins / isolation & purification*
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Neurofilament Proteins
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Peptide Fragments / isolation & purification*
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Skatole / analogs & derivatives
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Sodium Dodecyl Sulfate
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Thiocyanates
Substances
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Formates
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Hydroxylamines
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Intermediate Filament Proteins
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Neurofilament Proteins
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Peptide Fragments
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Thiocyanates
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formic acid
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BNPS-skatole
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Hydroxylamine
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Sodium Dodecyl Sulfate
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Skatole
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2-nitro-5-thiocyanobenzoic acid
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Cyanogen Bromide