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J Comp Neurol. 2019 Oct 1;527(14):2215-2232. doi: 10.1002/cne.24673. Epub 2019 Mar 20.

Gad1-promotor-driven GFP expression in non-GABAergic neurons of the nucleus endopiriformis in a transgenic mouse line.

Author information

1
Department of Physiological Genomics, Biomedical Center, Ludwig-Maximilians-University, Munich, Germany.
2
Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and University Hospital of Cologne, Cologne, Germany.
3
Center for Molecular Medicine (CMMC), University of Cologne, Cologne, Germany.

Abstract

Transgenic animals have become a widely used model to identify and study specific cell types in whole organs. Promotor-driven reporter gene labeling of the cells under investigation has promoted experimental efficacy to a large degree. However, rigorous assessment of transgene expression specificity in these animal models is highly recommended to validate cellular identity and to isolate potentially mislabeled cell populations. Here, we report on one such mislabeled neuron population in a widely used transgenic mouse line in which GABAergic somatostatin-expressing interneurons (SOMpos INs) are labeled by eGFP (so-called GIN mouse, FVB-Tg(GadGFP)45704Swn/J). These neurons represent a subpopulation of all SOMpos INs. However, we report here on GFP labeling of non-GABAergic neurons in the nucleus endopiriformis of this mouse line.

KEYWORDS:

GABA; GAD67-promotor; RRID:AB_10000343; RRID:AB_10562708; RRID:AB_10563568; RRID:AB_10563770; RRID:AB_11180061; RRID:AB_2057032; RRID:AB_2255365; RRID:AB_2307443; RRID:AB_2338013; RRID:AB_2338078; RRID:AB_2338251; RRID:AB_2338680; RRID:AB_2338928; RRID:AB_258984; RRID:AB_310288; RRID:AB_477019; eGFP; interneurons; nucleus endopiriformis; off-target labeling

PMID:
30847931
DOI:
10.1002/cne.24673

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