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Cancer Chemother Pharmacol. 2019 May;83(5):905-910. doi: 10.1007/s00280-019-03786-6. Epub 2019 Feb 13.

Determination of melphalan in human plasma by UPLC-UV method.

Author information

1
Drug Research Unit, Department of Clinical Pharmacy, School of Pharmacy, University of California at San Francisco, San Francisco, CA, 94110, USA. liusheng.huang@ucsf.edu.
2
Drug Research Unit, Department of Clinical Pharmacy, School of Pharmacy, University of California at San Francisco, San Francisco, CA, 94110, USA.
3
Department of Clinical Pharmacy, University of California at San Francisco, San Francisco, USA.
4
Department of Pediatrics, University of California at San Francisco, San Francisco, USA.

Abstract

It is desirable to develop a fast method for quantification of melphalan due to its instability. Here we report a method for quantification of melphalan (MPL) in human plasma using a UPLC-PDA system. Briefly, 50 µL plasma sample was mixed with 25 µL internal standard (2500 ng/mL acetylmelphalan in methanol) and 25 µL 20% trichloroacetic acid, and centrifuged at 21,000 g (15,000 rpm) at 4 °C for 3 min. The supernatant (5 µL) was injected onto an Acquity™ BEH C18 LC column (2.1 × 50 mm, 1.7 µm) and eluted with 25 mM NH4AC (pH 4.7)-acetonitrile in a gradient mode at a flow rate of 0.6 mL/min. The column kept at 40 ± 5 °C and the autosampler kept at 4 ± 5 °C. The detector set at 261 nm, and sampling rate was 40points/sec. The retention times were typically 2.11 min for melphalan and 2.38 min for the internal standard. Total run time is 4 min per sample. Calibration range was 100-40,000 ng/mL. The lower limit of quantification was 100 ng/mL. The method was validated based on the FDA guidelines, and applied to a clinical pharmacokinetic study in pediatric patients.

KEYWORDS:

Melphalan; Pediatric; Pharmacokinetic.; Plasma; UPLC–UV

PMID:
30847504
PMCID:
PMC6490959
[Available on 2020-05-01]
DOI:
10.1007/s00280-019-03786-6

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