Format

Send to

Choose Destination
Development. 2019 Mar 7;146(5). pii: dev172361. doi: 10.1242/dev.172361.

Kidney micro-organoids in suspension culture as a scalable source of human pluripotent stem cell-derived kidney cells.

Author information

1
Murdoch Children's Research Institute, Flemington Rd, Parkville, Victoria 3052, Australia.
2
Department of Paediatrics, The University of Melbourne, Parkville, Victoria 3010, Australia.
3
Department of Anatomy and Neuroscience, The University of Melbourne, Parkville, Victoria 3010, Australia.
4
School of Biosciences, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia.
5
Murdoch Children's Research Institute, Flemington Rd, Parkville, Victoria 3052, Australia melissa.little@mcri.edu.au.

Abstract

Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine. However, novel cost-effective techniques are needed to enable scaled-up production of kidney cell types in vitro We describe here a modified suspension culture method for the generation of kidney micro-organoids from human pluripotent stem cells. Optimisation of differentiation conditions allowed the formation of micro-organoids, each containing six to ten nephrons that were surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method. This suspension culture micro-organoid methodology resulted in a three- to fourfold increase in final cell yield compared with static culture, thereby representing an economical approach to the production of kidney cells for various biological applications.

KEYWORDS:

Kidney; Kidney micro-organoid; Nephron; Organoid; Pluripotent stem cell; Single cell profiling; Suspension culture

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center