Format

Send to

Choose Destination
Chem Sci. 2018 Nov 28;10(6):1709-1715. doi: 10.1039/c8sc03305f. eCollection 2019 Feb 14.

Sensitively distinguishing intracellular precursor and mature microRNA abundance.

Yang F1,2, Cheng Y1,2, Cao Y1,2, Dong H1,2, Lu H1,2, Zhang K1,2, Meng X1,2, Liu C1,2, Zhang X1,2.

Author information

1
Beijing Advanced Innovation Center for Materials Genome Engineering , University of Science and Technology Beijing , 30 Xueyuan Road , Beijing 100083 , P. R. China . Email: hfdong@ustb.edu.cn ; Email: zhangxueji@ustb.edu.cn.
2
Beijing Key Laboratory for Bioengineering and Sensing Technology , Research Center for Bioengineering and Sensing Technology , School of Chemistry & Biological Engineering , University of Science & Technology Beijing , 30 Xueyuan Road , Beijing 100083 , P. R. China.

Abstract

Mature microRNAs (miRNAs) produced from precursor microRNAs (pre-miRNAs) by the RNase Dicer have showed significant potential for cancer diagnosis and prognosis due to their key regulatory roles in various pathological processes. However, discriminatory detection of low-abundance miRNAs and pre-miRNAs remains a key challenge since the mature sequence is also present in the pre-miRNA forms. Herein, we report a novel cascade reaction to sensitively distinguish miRNAs versus pre-miRNAs in living cells based on two pairs of programmable hairpin oligonucleotide probes with a simple sequence design. The programmable hairpin probes can metastably coexist until the introduction of miRNAs or pre-miRNAs, which can trigger a specific hybridization chain reaction (HCR), respectively, leading to the self-assembly of nicked DNA duplex structures and a remarkable specific fluorescence intensity increase. The system can readily and sensitively assess the miRNA or pre-miRNA abundance in a homogeneous solution. The intracellular miRNA and pre-miRNA expression level assessment in different living cells is realized. Thus, we provide a novel investigation tool for discriminatorily and accurately assessing miRNA and pre-miRNA abundance, which could be useful for the biomedical application of miRNAs.

Supplemental Content

Full text links

Icon for Royal Society of Chemistry Icon for PubMed Central
Loading ...
Support Center