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Nutrients. 2019 Mar 5;11(3). pii: E548. doi: 10.3390/nu11030548.

Anti-Inflammatory Effects of Pomegranate Peel Extracts on In Vitro Human Intestinal Caco-2 Cells and Ex Vivo Porcine Colonic Tissue Explants.

Author information

1
Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, 01100 Viterbo, Italy. fabio.m@unitus.it.
2
School of Veterinary Medicine, University College Dublin 4, D04 V1W8 Dublin, Ireland. fabio.m@unitus.it.
3
School of Veterinary Medicine, University College Dublin 4, D04 V1W8 Dublin, Ireland. anindya131283@gmail.com.
4
Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, 01100 Viterbo, Italy. nicgio@unitus.it.
5
School of Veterinary Medicine, University College Dublin 4, D04 V1W8 Dublin, Ireland. marion.ryan@ucd.ie.
6
Department of Statistics, Computing, Applications "G. Parenti" (DISIA), PHYTOLAB, University of Florence, 50019 Florence, Italy. annalisa.romani@unifi.it.
7
Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, 01100 Viterbo, Italy. roberta.bernini@unitus.it.
8
School of Veterinary Medicine, University College Dublin 4, D04 V1W8 Dublin, Ireland. torres.sweeney@ucd.ie.

Abstract

The aim of this study was to determine the anti-inflammatory potential of pomegranate peel extracts (PPE) prepared from waste material of pomegranate juice production both in vitro on Caco-2 cells and ex vivo using porcine colonic tissue explants. Caco-2 cells were stimulated in vitro by TNF and colonic tissue explants were stimulated ex vivo with lipopolysaccharide (LPS). Both tissues were co-treated with PPE at 0, 1.0, 2.5, 5.0, 10 and 25 μg/mL. The secretion of CXCL8 in the supernatant of both experiments was determined by enzyme linked immunosorbent assay (ELISA) and the relative expression of inflammatory cytokines were evaluated in the colonic tissue by quantitative polymerase chain reaction (QPCR). The 2.5 to 25 μg/mL of PPE suppressed CXCL8 (p < 0.001) in the Caco-2 cells, whereas CXCL8 production was suppressed by only 5 and 25 μg/mL (p < 0.01) of PPE in the colonic explants. The 5 μg/mL of PPE also suppressed the expression of IL1A (p < 0.05), IL6 (p < 0.01) and CXCL8 (p < 0.05) in LPS challenged colonic tissues compared to controls. In conclusion, the 5 μg/mL of PPE consistently elicits strong anti-inflammatory activity. These results support the potential of bioactive compounds from the waste peel of pomegranate in terms of their anti-inflammatory activity in cells and tissues of the gastrointestinal tract.

KEYWORDS:

anti-inflammatory effects; circular economy; human colonic adenocarcinoma cells (Caco-2 cells); polyphenols; pomegranate (Punica granatum L.) peel extracts; porcine colonic tissues; punicalagin

PMID:
30841512
PMCID:
PMC6471410
DOI:
10.3390/nu11030548
[Indexed for MEDLINE]
Free PMC Article

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