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J Cell Sci. 2019 Apr 8;132(7). pii: jcs226860. doi: 10.1242/jcs.226860.

DSS-induced damage to basement membranes is repaired by matrix replacement and crosslinking.

Author information

1
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37240-7935, USA.
2
Center for Matrix Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
3
Program in Developmental Biology, Vanderbilt University, Nashville, TN 37232, USA.
4
Epithelial Biology Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
5
Division of Nephrology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
6
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235-1631, USA.
7
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37240-7935, USA andrea.page-mccaw@vanderbilt.edu.
8
Program in Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

Abstract

Basement membranes are an ancient form of animal extracellular matrix. As important structural and functional components of tissues, basement membranes are subject to environmental damage and must be repaired while maintaining functions. Little is known about how basement membranes get repaired. This paucity stems from a lack of suitable in vivo models for analyzing such repair. Here, we show that dextran sodium sulfate (DSS) directly damages the gut basement membrane when fed to adult Drosophila DSS becomes incorporated into the basement membrane, promoting its expansion while decreasing its stiffness, which causes morphological changes to the underlying muscles. Remarkably, two days after withdrawal of DSS, the basement membrane is repaired by all measures of analysis. We used this new damage model to determine that repair requires collagen crosslinking and replacement of damaged components. Genetic and biochemical evidence indicates that crosslinking is required to stabilize the newly incorporated repaired Collagen IV rather than to stabilize the damaged Collagen IV. These results suggest that basement membranes are surprisingly dynamic.

KEYWORDS:

Basement membrane; Collagen IV; Dextran sodium sulfate; Drosophila; Enterocytes; Matrix stiffness; Midgut

PMID:
30837285
PMCID:
PMC6467488
[Available on 2020-04-01]
DOI:
10.1242/jcs.226860

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