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Bioengineered. 2019 Dec;10(1):1-12. doi: 10.1080/21655979.2019.1586056.

MicroRNA-485-5p suppresses the proliferation, migration and invasion of small cell lung cancer cells by targeting flotillin-2.

Author information

1
a Department of Thoracic Surgery , the Fourth Hospital of Hebei Medical University , Shijiazhuang , China.
2
b Department of Clinical Laboratory , Hebei Medical University , Shijiazhuang , China.

Abstract

This study is aimed to elucidate the mechanisms underlying the role of miR-485-5p in small cell lung cancer (SCLC). The expression of miR-485-5p were quantified with real time quantitative PCR and it was found that the level of miR-485-5p was lower in SCLC tissues than normal tissues. In cultured SCLC cell lines, overexpression of miR-485-5p reduced cell proliferation, migration, and invasion in vitro, whereas knockdown of miR-485-5p performed contrary. FLOT2 expression was obviously upregulated and negatively correlated with miR-485-5p expression level in SCLC tissues. Overexpression of miR-485-5p significantly inhibited the protein expression of flotillin-2 (FLOT2) in cultured SCLC cells. Luciferase reporter assay confirmed that FLOT2 was a direct target of miR-485-5p in SCLC cells. It is concluded that miR-485-5p, as a tumor suppressor, inhibits the growth and metastasis in SCLC by targeting FLOT2. Upregulation of miR-485-5p expression may be an attractive strategy for SCLC therapy.

KEYWORDS:

FLOT2; metastasis; miR-485-5p; proliferation; small cell lung cancer

PMID:
30836864
PMCID:
PMC6527069
[Available on 2020-03-05]
DOI:
10.1080/21655979.2019.1586056

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