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Genes (Basel). 2019 Mar 4;10(3). pii: E196. doi: 10.3390/genes10030196.

Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains.

Author information

1
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK. phoebe.oldach@univ.ox.ac.uk.
2
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK. conrad.nieduszynski@path.ox.ac.uk.

Abstract

3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1.

KEYWORDS:

Chromatin interactions 4; Cohesin 2; DNA; Genome architecture 3; Replication timing 1

PMID:
30836708
DOI:
10.3390/genes10030196
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