Format

Send to

Choose Destination
Gene. 2019 May 25;698:113-119. doi: 10.1016/j.gene.2019.02.040. Epub 2019 Mar 2.

The expression of TET3 regulated cell proliferation in HepG2 cells.

Author information

1
Laboratory Animal Center, College of Animal Science, Jilin University, Changchun 130062, China.
2
Department of Emergency, First Hospital, Jilin University, Changchun 130031, Jilin, China.
3
Laboratory Animal Center, College of Animal Science, Jilin University, Changchun 130062, China. Electronic address: wang_dong_xu@jlu.edu.cn.

Abstract

Ten-eleven translocation (TET) proteins have been shown to be abnormally expressed in different cancers. To investigate the expression pattern of TET proteins in HepG2 cells, sodium ascorbate was used to treat HepG2 cells. Our results showed that TET1, TET2 and TET3 expression was increased after sodium ascorbate treatment. The TET proteins catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), thus, 5mC and 5hmC levels were examined. The results suggested that 5hmC was increased after sodium ascorbate treatment. To further determine the biological function of the TET proteins, si-TET1, si-TET2 and si-TET3 were transfected into HepG2 cells. The results showed that a knock down of TET3 expression stimulated cell proliferation of HepG2 cells. To further understand the effects of TET3 expression on cell proliferation, sodium ascorbate was added to the cells after transfection with si-TET3. The results demonstrated that sodium ascorbate could rescue TET3 expression and inhibit cell proliferation. Taken together, these results indicate that TET3 expression regulated cell proliferation, which is associated with 5hmC in HepG2 cells.

KEYWORDS:

5hmC; Cell proliferation; Gene expression; RNAi; TET3

PMID:
30836118
DOI:
10.1016/j.gene.2019.02.040
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center