Format

Send to

Choose Destination
Adv Healthc Mater. 2019 Apr;8(7):e1801631. doi: 10.1002/adhm.201801631. Epub 2019 Mar 5.

3D Bioprinting of Functional Islets of Langerhans in an Alginate/Methylcellulose Hydrogel Blend.

Author information

1
Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Faculty of Medicine of Technische Universität Dresden, Fetscherstr. 74, 01307, Dresden, Germany.
2
Paul Langerhans Institute Dresden of Helmholtz Centre Munich at University Hospital Carl Gustav Carus of Technische Universität Dresden and German Centre for Diabetes Research, Dresden, Tatzberg 47-49, 01307, Dresden, Germany.
3
Department of Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307, Dresden, Germany.

Abstract

Transplantation of pancreatic islets is a promising strategy to alleviate the unstable blood-glucose control that some patients with diabetes type 1 exhibit and has seen many advances over the years. Protection of transplanted islets from the immune system can be accomplished by encapsulation within a hydrogel, the most investigated of which is alginate. In this study, islet encapsulation is combined with 3D extrusion bioprinting, an additive manufacturing method which enables the fabrication of 3D structures with a precise geometry to produce macroporous hydrogel constructs with embedded islets. Using a plottable hydrogel blend consisting of clinically approved ultrapure alginate and methylcellulose (Alg/MC) enables encapsulating pancreatic islets in macroporous 3D hydrogel constructs of defined geometry while retaining their viability, morphology, and functionality. Diffusion of glucose and insulin in the Alg/MC hydrogel is comparable to diffusion in plain alginate; the embedded islets continuously produce insulin and glucagon throughout the observation and still react to glucose stimulation albeit to a lesser degree than control islets.

KEYWORDS:

3D bioprinting; alginate; diabetes; insulin; macroporous; pancreatic islet

PMID:
30835971
DOI:
10.1002/adhm.201801631

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center