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Stem Cell Res. 2019 Jan 26;36:101391. doi: 10.1016/j.scr.2019.101391. [Epub ahead of print]

Establishment of TUSMi008-A, an induced pluripotent stem cell (iPSC) line from a 76-year old Alzheimer's disease (AD) patient with PAXIP1 gene mutation.

Author information

1
Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, China; Lab of stem cell and neurodegeneration, Tongji University School of Medicine, Shanghai, China; Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
2
Department of Neurology, Shanghai East Hospital, China.
3
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
4
Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, China.
5
Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, China; Lab of stem cell and neurodegeneration, Tongji University School of Medicine, Shanghai, China. Electronic address: Jzhao@sibs.ac.cn.

Abstract

A 76-year old Alzheimer's disease (AD) female patient donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.

PMID:
30831521
DOI:
10.1016/j.scr.2019.101391
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