Format

Send to

Choose Destination
J Nutr Biochem. 2019 Jan 30;67:1-13. doi: 10.1016/j.jnutbio.2019.01.011. [Epub ahead of print]

Prebiotics from acorn and sago prevent high-fat-diet-induced insulin resistance via microbiome-gut-brain axis modulation.

Author information

1
Department of Internal Medicine-Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA; Department of Microbiology and Immunology, Wake Forest School of Medicine, Winston-Salem, NC, USA; Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
2
Department of Internal Medicine-Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA; Department of Microbiology and Immunology, Wake Forest School of Medicine, Winston-Salem, NC, USA.
3
National Center for the Biotechnology Workforce, Forsyth Technical Community College, Winston-Salem, NC, USA.
4
Gerontology and Geriatric Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA.
5
Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
6
Department of Internal Medicine-Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA; Department of Microbiology and Immunology, Wake Forest School of Medicine, Winston-Salem, NC, USA. Electronic address: hyadav@wakehealth.edu.

Abstract

Role of gut microbiome in obesity and type 2 diabetes (T2D) became apparent from several independent studies indicating that gut microbiome modulators like prebiotics may improve microbiome perturbations (dysbiosis) to ameliorate metabolic derangements. We herein isolate water soluble, nondigestible polysaccharides from five plant-based foods (acorn, quinoa, sunflower, pumpkin seeds and sago) and assess their impact on human fecal microbiome and amelioration of high-fat-diet (HFD)-induced obesity/T2D in mice. During polysaccharide isolation, purification, biochemical and digestion resistance characterization, and fermentation pattern by human fecal microbiome, we select acorn- and sago-derived prebiotics (on the basis of relatively higher purity and yield and lower protein contamination) and examine their effects in comparison to inulin. Prebiotics treatments in human fecal microbiome culture system not only preserve microbial diversity but also appear to foster beneficial bacteria and short-chain fatty acids (SCFAs). Feeding of acorn- and sago-derived prebiotics ameliorates HFD-induced glucose intolerance and insulin resistance in mice, with effects comparatively superior to those seen in inulin-fed mice. Feeding of both of novel prebiotics as well as inulin increases SCFAs levels in the mouse gut. Interestingly, gut hyperpermeability and mucosal inflammatory markers were significantly reduced upon prebiotics feeding in HFD-fed mice. Hypothalamic energy signaling in terms of increased expression of pro-opiomelanocortin was also modulated by prebiotics administration. Results demonstrate that these (and/or such) novel prebiotics can ameliorate HFD-induced defects in glucose metabolism via positive modulation of gut-microbiome-brain axis and hence could be useful in preventing/treating diet-induced obesity/T2D.

KEYWORDS:

Diabetes; Fibers; Metabolites; Microbiome; Obesity; Polysaccharides; Prebiotic

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center