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Clin Exp Dermatol. 2019 Dec;44(8):882-892. doi: 10.1111/ced.13959. Epub 2019 Apr 24.

Transcriptome patterns in hidradenitis suppurativa: support for the role of antimicrobial peptides and interferon pathways in disease pathogenesis.

Author information

1
Division of Rheumatology, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.
2
Computational Biology Institute, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
3
Department of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.

Abstract

BACKGROUND:

Hidradenitis suppurativa (HS) is a recurrent inflammatory disease of the apocrine sweat glands. Immune dysregulation probably contributes to the pathogenesis of HS.

AIM:

To harness mRNA expression arrays to investigate the transcriptome profile in HS compared with control skin.

METHODS:

Illumina® HumanHT-12 v4 Expression BeadChips were used to measure mRNA expression in skin samples from HS (n = 10) and abdominoplasty (n = 11) skin specimens. Differentially expressed genes were detected by fitting genewise linear models to the normalized expression data and then modelling using the web-based software Ingenuity® Pathway Analysis.

RESULTS:

The antimicrobial peptide Dermcidin and the cytokine regulator interleukin (IL)-37 were both significantly downregulated in the HS specimens (Dermcidin expression log ratio -3.93, expression P = 0.04; IL-37 expression log ratio -3.29, expression P < 0.001). Pathway analysis revealed the interferon-signalling pathway, leucocyte extravasation pathway, T helper 1 and 2 pathways and nuclear factor of activated T cells as the top-five upregulated pathways in the HS samples.

CONCLUSION:

Evaluation of transcriptome patterns in HS compared with normal skin demonstrated downregulation of the antimicrobial peptide Dermcidin and the innate immune regulator IL-37, as well as upregulation of interferon pathways and pathways of leucocyte activation.

PMID:
30828847
DOI:
10.1111/ced.13959

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