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Plant Pathol J. 2019 Feb;35(1):84-89. doi: 10.5423/PPJ.NT.09.2018.0193. Epub 2019 Feb 1.

Genome-wide Screening to Identify Responsive Regulators Involved in the Virulence of Xanthomonas oryzae pv. oryzae.

Han SW1, Lee MA2,3, Yoo Y2,3, Cho MH2, Lee SW2,3.

Author information

1
Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea.
2
Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea.
3
Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.

Abstract

Two-component systems (TCSs) are critical to the pathogenesis of Xanthomonas oryzae pv. oryzae (Xoo). We mutated 55 of 62 genes annotated as responsive regulators (RRs) of TCSs in the genome of Xoo strain PXO99A and identified 9 genes involved in Xoo virulence. Four (rpfG, hrpG, stoS, and detR) of the 9 genes were previously reported as key regulators of Xoo virulence and the other 5 have not been characterized. Lesion lengths on rice leaves inoculated with the mutants were shorter than those of the wild type and were significantly restored with gene complementation. The population density of the 5 mutants in planta was smaller than that of PXO99A at 14 days after inoculation, but the growth curves of the mutants in rich medium were similar to those of the wild type. These newly reported RR genes will facilitate studies on the function of TCSs and of the integrated regulation of TCSs for Xoo pathogenesis.

KEYWORDS:

Xanthomonas; response regulator; two-component regulatory system

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