Format

Send to

Choose Destination
Vet Microbiol. 2019 Mar;230:241-243. doi: 10.1016/j.vetmic.2019.02.004. Epub 2019 Feb 4.

Detection of antibodies to Borrelia burgdorferi s.l. in wild small mammals and sensitivity of PCR and cultivation.

Author information

1
Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic.
2
Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic; Department of Biology, Faculty of Education, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic.
3
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42 Brno, Czech Republic.
4
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42 Brno, Czech Republic. Electronic address: bartovae@vfu.cz.

Abstract

The aim of this study was to determine the seroprevalence of antibodies to B. burgdorferi s.l. in wild small mammals in the Czech Republic and compare sensitivity of PCR and cultivaton. Wild small mammals (n = 691) were trapped in years 2010-2014 in three localities of the Czech Republic. Heart rinses (n = 340) and sera (n = 351) were examined by modified indirect ELISA. Seventy animals were randomly selected for comparison of results of cultivation and PCR. Mean annual antiborelian positivity was 12% with statistical difference (p < 0.05) between Bank Vole (Clethrionomys glareolus) and other six animal species, while there was no statistical difference (p > 0.05) between rodentia and insectivora, gender and localities. The cultivation revealed one positive sample (1.4%), negative in both PCR and ELISA. Method PCR revealed seven positive samples (10%); two of them were simultaneously dubious in ELISA. Eleven animals, negative in cultivation and PCR, had antibodies in ELISA. Method of PCR compared to cultivation seems to be more sensitive for detection of Borrelia.

KEYWORDS:

Lyme borreliosis; Rodents; Seroprevalence; Spirochetes; Ticks; Vector-borne disease

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center