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Neurosci Lett. 2019 May 14;701:226-233. doi: 10.1016/j.neulet.2019.02.038. Epub 2019 Feb 28.

miR-9-5p modulates the progression of Parkinson's disease by targeting SIRT1.

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Rehabitation Department, Yantai Hospital of Traditional Chinese Medicine, 264001, China.
Department of Neurology, Yantai Hospital of Traditional Chinese Medicine, 264001, China.
Department of Neurology, The Affiliated Hospital of Qingdao University, 266000, China.
Emergency Department, Yantai Hospital of Traditional Chinese Medicine, 264001, China. Electronic address:


Parkinson's disease (PD) is a most common progressive neurodegenerative disease mainly occurring in the elderly. Plenty of miRNAs are reported to involve in the progression of PD. However, the role of miR-9-5p in the regulation of PD pathogenesis remains unclear. The expressions of miR-9-5p and Sirtuin 1 (SIRT1) at mRNA and protein levels were determined by qRT-PCR and western blotting (WB) analyses. Cell viability and apoptosis were evaluated by MTT and flow cytometry. The levels of apoptosis-related proteins Bcl-2, Bax, Caspase-3 were detected by WB analysis. The releases of inflammatory cytokines IL-1β and TNF-α were examined by ELISA assay. ROS generation, LDH and SOD activity were evaluated using commercially available kits. Bioinformatics analysis, luciferase reporter, and qRT-PCR assays were performed to demonstrate the true interaction between miR-9-5p and SIRT1. Results showed miR-9-5p was upregulated and SIRT1 was downregulated in MPP+-treated SH-SY5Y cells in dose- and time- dependent manners. miR-9-5p knockdown attenuated MPP+-induced neurotoxicity in SH-SY5Y cells, as evidenced by the enhancement in cell viability, and the suppression in cell apoptosis, inflammation, and oxidative stress. SIRT1 was identified to be a target of miR-9-5p. Restoration of miR-9-5p aggravated SIRT1-attenuated neurotoxicity in MPP+-treated SH-SY5Y cells. Our data suggested these data indicated that miR-9-5p exerted a neurotoxic role in MPP+-derived PD by directly targeting STAT1, providing a potential therapeutic strategy for patients troubled by PD.


Neurotoxicity; Parkinson’s disease; Sirtuin 1; miR-9-5p

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