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Mycoses. 2019 Mar 2. doi: 10.1111/myc.12908. [Epub ahead of print]

Comparison of genotyping methods for Cunninghamella bertholletiae.

Author information

1
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
2
FG 16: Erreger von Pilz-, und Parasiteninfektionen und Mykobakteriosen, Konsiliarlabor für Kryptokokkose und seltene Systemmykosen, Robert Koch-Institut, Berlin, Germany.
3
Department I of Internal Medicine, University Hospital of Cologne, Cologne, Germany.
4
Clinical Microbiology Department, Hospital Universitario La Paz, Madrid, Spain.
5
Institute of Clinical Hygiene, Medical Microbiology and Infectiology, Hospital Nuremberg, Paracelsus Medical University, Nuremberg, Germany.

Abstract

BACKGROUND:

Invasive fungal infections caused by filamentous fungi of the order Mucorales are serious complications in immunocompromised patients and often associated with fatal outcome. As a member of this order, Cunninghamella bertholletiae is a saprophytic fungus with naturally exhibited high minimum inhibitory concentrations against common antifungal drugs and with the potential for outbreaks in clinical settings.

OBJECTIVES AND METHODS:

In a proof of principle study, we evaluated the performance of microsatellite markers for the discrimination of thirteen C. bertholletiae isolates from various sources in comparison with a repetitive sequence-based PCR (rep-PCR) and random amplification of polymorphic DNA (RAPD). Based on the higher discriminatory power of the microsatellite PCR with five separate primer pairs [Simpson's Index of 1 vs. 0 (RAPD) and 0 (rep-PCR)], the novel method was applied to eight additional isolates, including four well-characterized isolates from a cluster of infections in a next step.

RESULTS:

In total, microsatellite PCR identified 21 separate genotypes. A probable epidemiological association of the cluster isolates could be demonstrated by microsatellite genotyping.

CONCLUSION:

In conclusion, our findings demonstrate the value of microsatellite PCR in genotyping Cunninghamella bertholletiae and its potential for future applications with other species of the order Mucorales. This article is protected by copyright. All rights reserved.

KEYWORDS:

Cunninghamella; mucormycosis; multilocus microsatellite PCR; nosocomial outbreaks; typing

PMID:
30825344
DOI:
10.1111/myc.12908

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