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Sci Immunol. 2019 Mar 1;4(33). pii: eaaw1941. doi: 10.1126/sciimmunol.aaw1941.

Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors.

Author information

1
Immunobiology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. caetano@crick.ac.uk mar.cabeza-cabrerizo@crick.ac.uk jannekevanblijswijk@gmail.com frederick.klauschen@charite.de.
2
Institute of Pathology, Charité University Medicine Berlin, Charitéplatz 1, D-10117 Berlin, Germany.
3
Tumour Cell Biology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
4
Bioinformatics, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
5
Immunobiology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
6
Hubrecht Institute, Uppsalalaan 8, 3584 CT Utrecht, Netherlands.
7
Aix-Marseille University Centre, National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Immunologie de Marseille-Luminy (CIML), Marseille, France.
8
Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
9
Institute of Pathology, Charité University Medicine Berlin, Charitéplatz 1, D-10117 Berlin, Germany. caetano@crick.ac.uk mar.cabeza-cabrerizo@crick.ac.uk jannekevanblijswijk@gmail.com frederick.klauschen@charite.de.

Abstract

Conventional dendritic cells (cDCs) are found in all tissues and play a key role in immune surveillance. They comprise two major subsets, cDC1 and cDC2, both derived from circulating precursors of cDCs (pre-cDCs), which exited the bone marrow. We show that, in the steady-state mouse, pre-cDCs entering tissues proliferate to give rise to differentiated cDCs, which themselves have residual proliferative capacity. We use multicolor fate mapping of cDC progenitors to show that this results in clones of sister cDCs, most of which comprise a single cDC1 or cDC2 subtype, suggestive of pre-cDC commitment. Upon infection, a surge in the influx of pre-cDCs into the affected tissue dilutes clones and increases cDC numbers. Our results indicate that tissue cDCs can be organized in a patchwork of closely positioned sister cells of the same subset whose coexistence is perturbed by local infection, when the bone marrow provides additional pre-cDCs to meet increased tissue demand.

PMID:
30824528
PMCID:
PMC6420147
[Available on 2019-09-01]
DOI:
10.1126/sciimmunol.aaw1941

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