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Plant Sci. 2019 Mar;280:321-329. doi: 10.1016/j.plantsci.2018.12.019. Epub 2018 Dec 21.

GRA78 encoding a putative S-sulfocysteine synthase is involved in chloroplast development at the early seedling stage of rice.

Author information

1
Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
2
Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: xjdeng2006@aliyun.com.
3
Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: prwang@sicau.edu.cn.

Abstract

Cysteine functions not only as an amino acid in proteins but also as a precursor for a large number of essential biomolecules. Cysteine is synthesized via the incorporation of sulfide to O-acetylserine under the catalysis of O-acetylserine(thiol)lyase (OASTL). In dicotyledonous Arabidopsis, nine OASTL genes have been reported. However, in their null mutants, only the mutant of CS26 encoding S-sulfocysteine synthase showed the visible phenotypic changes, displaying significantly small plants and pale-green leaves under long-day condition but not short-day condition. Up to now, no OASTL gene or mutant has been identified in monocotyledon. In this study, we isolated a green-revertible albino mutant gra78 in rice (Oryza sativa). Its albino phenotype at the early seedling stage was sensitive to temperature but independent of photoperiod. Map-based cloning revealed that candidate gene LOC_Os01g59920 of GRA78 encodes a putative S-sulfocysteine synthase showing significant similarity with Arabidopsis CS26. Complementation experiment confirmed that mutation in LOC_Os01g59920 accounted for the mutant phenotype of gra78. GRA78 is constitutively expressed in all tissues and its encoded protein is targeted to the chloroplast. In addition, qRT-PCR suggested that expression levels of four OASTL homolog genes and five photosynthetic genes were remarkably down-regulated.

KEYWORDS:

Cysteine biosynthesis; Green-revertible albino mutant; Map-based cloning; Rice; S-sulfocysteine synthase

PMID:
30824011
DOI:
10.1016/j.plantsci.2018.12.019
[Indexed for MEDLINE]

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