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Plant Sci. 2019 Mar;280:219-227. doi: 10.1016/j.plantsci.2018.09.021. Epub 2018 Sep 29.

OsNF-YC10, a seed preferentially expressed gene regulates grain width by affecting cell proliferation in rice.

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College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, School of Life Sciences, Nanchang University, Nanchang, 330031, China. Electronic address:
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:


Grain size and shape are important factors in determining the grain yield. In this study, OsNF-YC10, a member of the NF-Y transcription factor family encoding a putative histone transcription factor, was cloned and characterized. qRT-PCR and mRNA in situ hybridization analysis revealed that OsNF-YC10 was highly expressed in endosperm and spikelet hull at late developmental stages. The results showed that OsNF-YC10 was a nuclear protein showing transcription activation activity. The osnf-yc10 lines, produced using CRISPR/Cas9 technology, showed narrow, thin and light grains. Cytological experiments revealed significantly reduced cell number of spikelet hull in osnf-yc10 lines compared with that in WT. Narrow, thin, and light grains were found consistently in OsNF-YC10 RNAi transgenic lines. Moreover, the number of cells decreased in the grain-width direction than WT. These results indicated that OsNF-YC10 plays an important role in determining grain size and shape. OsNF-YC10 was further revealed to influence the expression of GW8 (a positive regulator of grain width), GW7 (a negative regulator of grain width) and cell cycle-regulated genes CYCD4, CYCA2.1, CYCB2.1, CYCB2.2, E2F2. Taken together, it is suggested that OsNF-YC10 regulates the grains size and shape by influencing the cell proliferation of spikelet hulls.


CRISPR and RNAi; agronomic traits; cell; grain size and shape; proliferation; spikelet hulls

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