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Plant Sci. 2019 Mar;280:219-227. doi: 10.1016/j.plantsci.2018.09.021. Epub 2018 Sep 29.

OsNF-YC10, a seed preferentially expressed gene regulates grain width by affecting cell proliferation in rice.

Author information

1
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
2
Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, School of Life Sciences, Nanchang University, Nanchang, 330031, China. Electronic address: wangxin@ncu.edu.cn.
3
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: yaojlmy@mail.hzau.edu.cn.

Abstract

Grain size and shape are important factors in determining the grain yield. In this study, OsNF-YC10, a member of the NF-Y transcription factor family encoding a putative histone transcription factor, was cloned and characterized. qRT-PCR and mRNA in situ hybridization analysis revealed that OsNF-YC10 was highly expressed in endosperm and spikelet hull at late developmental stages. The results showed that OsNF-YC10 was a nuclear protein showing transcription activation activity. The osnf-yc10 lines, produced using CRISPR/Cas9 technology, showed narrow, thin and light grains. Cytological experiments revealed significantly reduced cell number of spikelet hull in osnf-yc10 lines compared with that in WT. Narrow, thin, and light grains were found consistently in OsNF-YC10 RNAi transgenic lines. Moreover, the number of cells decreased in the grain-width direction than WT. These results indicated that OsNF-YC10 plays an important role in determining grain size and shape. OsNF-YC10 was further revealed to influence the expression of GW8 (a positive regulator of grain width), GW7 (a negative regulator of grain width) and cell cycle-regulated genes CYCD4, CYCA2.1, CYCB2.1, CYCB2.2, E2F2. Taken together, it is suggested that OsNF-YC10 regulates the grains size and shape by influencing the cell proliferation of spikelet hulls.

KEYWORDS:

CRISPR and RNAi; agronomic traits; cell; grain size and shape; proliferation; spikelet hulls

PMID:
30824000
DOI:
10.1016/j.plantsci.2018.09.021
[Indexed for MEDLINE]

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