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Plant Sci. 2019 Mar;280:12-17. doi: 10.1016/j.plantsci.2018.10.028. Epub 2018 Nov 13.

Sphingolipid-induced cell death in Arabidopsis is negatively regulated by the papain-like cysteine protease RD21.

Author information

1
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France. Electronic address: ormancey@lrsv.ups-tlse.fr.
2
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France. Electronic address: thuleau@lrsv.ups-tlse.fr.
3
The Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK. Electronic address: renier.vanderhoorn@plants.ox.ac.uk.
4
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France. Electronic address: grat@lrsv.ups-tlse.fr.
5
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France. Electronic address: testard@lrsv.ups-tlse.fr.
6
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France; Agronomy Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt. Electronic address: kymostafa@agri.zu.edu.eg.
7
Institute of Plant Sciences Paris Saclay (IPS2), CNRS, INRA, Université Paris-Sud, Université d'Evry Val d'Essonne, Université Paris-Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 630, Plateau du Moulon, 91192, Gif-sur-Yvette, France. Electronic address: marie.boudsocq@cnrs.fr.
8
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France. Electronic address: cotelle@lrsv.ups-tlse.fr.
9
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP42617, 31326, Castanet Tolosan, France. Electronic address: mazars@lrsv.ups-tlse.fr.

Abstract

It is now well established that sphingoid Long Chain Bases (LCBs) are crucial mediators of programmed cell death. In plants, the mycotoxin fumonisin B1 (FB1) produced by the necrotrophic fungus Fusarium moniliforme disrupts the sphingolipid biosynthesis pathway by inhibiting the ceramide synthase leading to an increase in the amount of phytosphingosine (PHS) and dihydrosphingosine (DHS), the two major LCBs in Arabidopsis thaliana. To date, the signaling pathway involved in FB1-induced cell death remains largely uncharacterized. It is also well acknowledged that plant proteases such as papain-like cysteine protease are largely involved in plant immunity. Here, we show that the papain-like cysteine protease RD21 (responsive-to-desiccation-21) is activated in response to PHS and FB1 in Arabidopsis cultured cells and leaves, respectively. Using two allelic null mutants of RD21, and two different PCD bioassays, we demonstrate that the protein acts as a negative regulator of FB1-induced cell death in Arabidopsis.

KEYWORDS:

Activity-based protein profiling; Fumonisin B1; Papain-like cysteine proteases; Programmed cell death; RD21; Sphingolipids

PMID:
30823989
DOI:
10.1016/j.plantsci.2018.10.028
[Indexed for MEDLINE]

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