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Glycoconj J. 2019 Feb 28. doi: 10.1007/s10719-019-09861-7. [Epub ahead of print]

AGEs induce epithelial to mesenchymal transformation of human peritoneal mesothelial cells via upregulation of STAT3.

Author information

1
Department of Nephrology, The First Affiliated Hospital of Anhui Medical University, No 218, Jixi Road, Hefei, 230022, Anhui, China. zhangpeiayk@163.com.
2
Department of Nephrology, The First Affiliated Hospital of Anhui Medical University, No 218, Jixi Road, Hefei, 230022, Anhui, China.
3
Institute of pharmacology, AnHui Medical University, AnHui, Hefei, 230032, China.

Abstract

Epithelial-mesenchymal transition (EMT) in peritoneum was induced during peritoneal dialysis (PD), which finally caused progressive fibrosis. However, the underlying mechanisms were not well elucidated. We established advanced glycation end-products (AGEs)-induced EMT model using primary human peritoneal mesothelial cells (HPMCs). The working concentration and time of AGEs were optimized. Then the expression and activation signal transducer and activator of transcription 3 (STAT3), a key factor of EMT in cancer, were detected. The regulation of STAT3 by miRNA was also explored. 50 μg/ml AGEs treatment for 24 h can successfully induce EMT in HPMCs. AGEs treatment upregulated and activated STAT3. miRNA-454, potentially targeting STAT3, was down-regulated in AGEs-treated HPMCs. Overexpression of miRNA-454 prevented AGEs- induced EMT in HPMCs. AGEs induce epithelial to mesenchymal transformation of Human peritoneal mesothelial cells via upregulation of STAT3.

KEYWORDS:

AGEs; EMT; HPMCs; STAT3; miRNA

PMID:
30820783
DOI:
10.1007/s10719-019-09861-7

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