Send to

Choose Destination
Sci Adv. 2019 Feb 27;5(2):eaav5447. doi: 10.1126/sciadv.aav5447. eCollection 2019 Feb.

Increased glymphatic influx is correlated with high EEG delta power and low heart rate in mice under anesthesia.

Author information

Center for Translational Neuromedicine, University of Rochester Medical Center, Rochester, NY 14642, USA.
Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Department of Clinical Pharmacology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.


The glymphatic system is responsible for brain-wide delivery of nutrients and clearance of waste via influx of cerebrospinal fluid (CSF) alongside perivascular spaces and through the brain. Glymphatic system activity increases during sleep or ketamine/xylazine (K/X) anesthesia, yet the mechanism(s) facilitating CSF influx are poorly understood. Here, we correlated influx of a CSF tracer into the brain with electroencephalogram (EEG) power, heart rate, blood pressure, and respiratory rate in wild-type mice under six different anesthesia regimens. We found that glymphatic CSF tracer influx was highest under K/X followed by isoflurane (ISO) supplemented with dexmedetomidine and pentobarbital. Mice anesthetized with α-chloralose, Avertin, or ISO exhibited low CSF tracer influx. This is the first study to show that glymphatic influx correlates positively with cortical delta power in EEG recordings and negatively with beta power and heart rate.

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center