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J Conserv Dent. 2019 Jan-Feb;22(1):23-27. doi: 10.4103/JCD.JCD_216_18.

Comparative evaluation of angiogenesis using a novel platelet-rich product: An in vitro study.

Author information

Department of Conservative and Endodontics, Noorul Islam College of Dental Science, Kerala University of Health Sciences, Thiruvananthapuram, Kerala, India.
Department of Biotechnology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India.



In vivo angiogenesis is normal and vital process in growth and development, wound healing, and formation of granulation tissue wherein new blood vessels form from preexisting vessels as part of revascularization. Platelet-rich products promote wound healing associated with angiogenesis. Biomaterials such as titanium were found to be angiogenic. Unlike in vivo situations, in vitro angiogenesis, study cells, within a controlled environment.


The aim of this study is to evaluate the angiogenic potential of a novel platelet-rich product.

Materials and Methods:

Blood was drawn from volunteers with informed consent. Blood samples were centrifuged to obtain platelet-rich products. Platelet concentrates prepared were platelet-rich plasma (PRP), platelet-rich fibrin, and a novel platelet-rich product which is titanium-prepared PRP (TPRP), obtained using titanium. The study which compared platelet concentrate was divided into four groups subjected to tissue culture. Phase-contrast microscope was used to determine the rate of growth by cell counting.

Statistical Analysis:

ANOVA was used for comparison within groups and post hoc for multiple comparisons.


TPRP group showed granular ground substance. Group with platelet-rich fibrin (PRF) shows a high rate of growth whereas those with TPRP showed better growth rate when compared to its counterpart, PRP.


This is the first study which introduces TPRP. Previous studies have proved that titanium-prepared PRF has better structural quality than its counterpart platelet-rich fibrin. This study concludes that TPRP has better angiogenic potential than its counterpart PRP. Further in vivo studies are needed to promote TPRP as a new generation of platelet products.


Angiogenesis; platelet-rich products; titanium

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