Send to

Choose Destination
J Conserv Dent. 2019 Jan-Feb;22(1):17-22. doi: 10.4103/JCD.JCD_272_18.

Cellular interaction and antibacterial efficacy of two hydraulic calcium silicate-based cements: Cell-dependent model.

Author information

Department of Dental Biomaterials, Faculty of Oral and Dental Surgery, Zagazig University, Zagazig, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Department of Oral Biology, Faculty of Oral and Dental Surgery, Zagazig University, Zagazig, Egypt.



This study investigated cytotoxic probability, osteogenic potential, and antibacterial efficacy of two pulp-capping hydraulic calcium-silicate cements.

Materials and Methods:

For osteogenic potential and cytotoxicity evaluation, mesenchymal stem cells (MSCs) and materials disc-shaped specimens were used. Increase or decrease in a number of proliferating MSCs was calculated after three intervals. Alkaline phosphatase (ALP) levels in osteogenic media were normalized to the total protein content of cells and measured spectrophotometrically. Antibacterial efficiency through growth curves of Streptococcus mutans in direct contact with tested materials.


Biodentine showed the highest number of proliferating MSCs (278000.41 ± 4000.06, after 72 h) and the highest concentration of ALP after 12 days (209.26 ± 7.17 μU/μg protein). It showed the lowest slope (0.003 ± 0.0005) of S. mutans strains growth curves after 18 h.


Biodentine proved a highly significant osteogenic ability and gave a significant reduction of S. mutans growth.


Antibacterial efficiency; cytotoxicity; hydraulic cements; osteogenic potential

Supplemental Content

Full text links

Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
Loading ...
Support Center