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Genes Dev. 2019 Apr 1;33(7-8):466-476. doi: 10.1101/gad.316554.118. Epub 2019 Feb 28.

Auxin regulates endosperm cellularization in Arabidopsis.

Author information

1
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, 7080 Uppsala, Sweden.
#
Contributed equally

Abstract

The endosperm is an ephemeral tissue that nourishes the developing embryo, similar to the placenta in mammals. In most angiosperms, endosperm development starts as a syncytium, in which nuclear divisions are not followed by cytokinesis. The timing of endosperm cellularization largely varies between species, and the event triggering this transition remains unknown. Here we show that increased auxin biosynthesis in the endosperm prevents its cellularization, leading to seed arrest. Auxin-overproducing seeds phenocopy paternal-excess triploid seeds derived from hybridizations of diploid maternal plants with tetraploid fathers. Concurrently, auxin-related genes are strongly overexpressed in triploid seeds, correlating with increased auxin activity. Reducing auxin biosynthesis and signaling reestablishes endosperm cellularization in triploid seeds and restores their viability, highlighting a causal role of increased auxin in preventing endosperm cellularization. We propose that auxin determines the time of endosperm cellularization, and thereby uncovered a central role of auxin in establishing hybridization barriers in plants.

KEYWORDS:

auxin; cellularization; endosperm; hybridization barrier; seed development; triploid block

PMID:
30819818
PMCID:
PMC6446538
DOI:
10.1101/gad.316554.118
[Indexed for MEDLINE]
Free PMC Article

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