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Food Sci Biotechnol. 2018 Aug 11;28(1):227-236. doi: 10.1007/s10068-018-0426-x. eCollection 2019 Feb.

Inhibitory effects of Orostachys malacophyllus var. iwarenge extracts on reactive oxygen species production and lipid accumulation during 3T3-L1 adipocyte differentiation.

Jang M#1,2, Choi HY#1,2, Kim GH1,2.

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1Plant Resources Research Institute, Duksung Women's University, Seoul, 132-714 Korea.
2Department of Food and Nutrition, Duksung Women's University, 419, Ssangmun-Dong, Dobong-Gu, Seoul, 132-714 Korea.
Contributed equally


Orostachys malacophyllus var. iwarenge was extracted with water, 30 and 70% ethyl alcohol. The ethyl alcohol extracts showed higher total phenol contents and greater antioxidant effects than the water extract. Treatment with the 70% alcohol extract inhibited reactive oxygen species (ROS) production and lipid accumulation during 3T3-L1 adipogenesis. Furthermore, the 70% extract inhibited the mRNA and protein expressions of the pro-oxidant enzyme NADPH oxidase 4 and of the NADPH-producing enzyme glucose-6-phosphate dehydrogenase. The mRNA and protein expressions of antioxidant enzymes, such as copper-zinc superoxide dismutase and manganese superoxide dismutase increased in cells treated with the 70% alcohol extract. In addition, this extract suppressed the mRNA and protein levels of adipogenic transcription factors and of their marker genes. These results indicate that O. malacophyllus extracts inhibit lipid accumulation and ROS production by controlling adipogenic factors and pro-/anti-oxidant enzyme responses.


3T3-L1 cells; Adipocyte differentiation; Antioxidant; Orostachys malacophyllus; Reactive oxygen species

[Available on 2019-08-11]

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