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J Glob Infect Dis. 2019 Jan-Mar;11(1):25-29. doi: 10.4103/jgid.jgid_28_18.

Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis.

Author information

1
Department of Obstetrics and Gynecology, Faculty of Medicine, Christian University of Indonesia, Jakarta, Indonesia.
2
Department of Microbiology, Molecular Biology and Immunology Laboratory and Faculty of Medicine, Hasanuddin University, Makassar,, Indonesia.
3
Department of Obstetric and Gynecology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
4
Department of Anaestesiology, Faculty of Medicine, Christian University of Indonesia, Jakarta, Indonesia.
5
Midwife Program of Medistra Health Higher School, Jakarta, Indonesia.
6
Midwife Program of Yaleka-Maro School, Merauke, Papua, Indonesia.
7
Department of Medical Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia.

Abstract

Background:

Curcuma longa has strong anti-inflammatory effect. This study aims to evaluated the level of anti-Toxoplasma immunoglobulin G and immunoglobulin M (IgG-IgM) antibody after intervention with C. longa extract in early pregnant mice with acute toxoplasmosis.

Materials and Methods:

We evaluated 20 early pregnant mice that were divided into five groups, four mice in each. Group 1-4 received injections of Toxoplasma gondii tachyzoites. Three days later, G1 and G2 were given orally 125 mg/kg/day and 500 mg/kg/day of C. longa extract, respectively. G3 was given 60 mg/kg/day of spiramycin (positive control), and G4 was given 0.2 ml of distilled water (negative control). G5 underwent no intervention at all. Blood samples were obtained serially (before and 3 days after injection of tachyzoites, 3 days and 7 days after intervention) to assess anti-Toxoplasma IgG-IgM antibody levels by enzyme-linked immunosorbent assay methods.

Results:

Anti-Toxoplasma IgG-IgM antibody levels increased significantly 3 days after injection of tachyzoites (P < 0.05), but decreased significantly (P < 0.05) 3 days, and 7 days after administration of C. longa extract dose 125 mg, 500 mg, and spiramycin 60 mg, and there was no significant difference between these three groups. Anti-Toxoplasma IgG-IgM antibody levels increased significantly (P < 0.05) 3 days, 6 days, and 10 days after injections of tachyzoites on G4. The IgG-IgM antibody levels fluctuated on G5 and considered as insignificant (P > 0.05).

Conclusion:

The administration of C. longa extract at a dose of 125 mg/kg/day for 7 days effectively decreased anti-Toxoplasma IgG-IgM antibody level in early pregnant mice with acute toxoplasmosis.

KEYWORDS:

Curcuma longa; early pregnancy; immunoglobulin G; immunoglobulin M; toxoplasmosis

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