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Sci Rep. 2019 Feb 27;9(1):2950. doi: 10.1038/s41598-019-39588-2.

The IKK-binding domain of NEMO is an irregular coiled coil with a dynamic binding interface.

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Department of Chemistry, Dartmouth College, Hanover, NH, 03755, USA.
Department of Chemistry, Dartmouth College, Hanover, NH, 03755, USA.


NEMO is an essential component in the activation of the canonical NF-κB pathway and exerts its function by recruiting the IκB kinases (IKK) to the IKK complex. Inhibition of the NEMO/IKKs interaction is an attractive therapeutic paradigm for diseases related to NF-κB mis-regulation, but a difficult endeavor because of the extensive protein-protein interface. Here we report the high-resolution structure of the unbound IKKβ-binding domain of NEMO that will greatly facilitate the design of NEMO/IKK inhibitors. The structures of unbound NEMO show a closed conformation that partially occludes the three binding hot-spots and suggest a facile transition to an open state that can accommodate ligand binding. By fusing coiled-coil adaptors to the IKKβ-binding domain of NEMO, we succeeded in creating a protein with improved solution behavior, IKKβ-binding affinity and crystallization compatibility, which will enable the structural characterization of new NEMO/inhibitor complexes.

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