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Jpn J Infect Dis. 2019 Feb 28. doi: 10.7883/yoken.JJID.2018.403. [Epub ahead of print]

Genome-based epidemiological analysis of 13 Acinetobacter strains isolated from blood cultures of hospitalized patients from a university hospital in Japan.

Author information

1
Department of Bacteriology, Osaka City University Graduate School of Medicine.
2
Research Center for Infectious Disease Sciences, Osaka City University Graduate School of Medicine.
3
Antimicrobial Resistance Research Center, National Institute of Infectious Diseases.
4
Department of Infection Control and Prevention, Osaka City University Hospital.
5
Department of Infection Control Science, Osaka City University Graduate School of Medicine.
6
Department of Respiratory Medicine, Osaka City University Graduate School of Medicine.
7
Department of Medical Technology, Morinomiya University of Medical Sciences.
8
Department of Laboratory Medicine, Osaka City University Hospital.
9
Department of Bacteriology II, National Institute of Infectious Diseases.

Abstract

The genus Acinetobacter comprises many species that can cause infectious diseases. Despite their importance as nosocomial pathogens, the clinical distributions of individual species or clones are not well understood. In this study, we aimed to characterize 13 Acinetobacter strains isolated from blood cultures from Osaka City University Hospital. We conducted whole-genome sequencing to reveal their genetic background. We also performed PCR-based open reading frame typing (POT) and compared the results with those of multilocus sequence typing (MLST) to confirm its reliability as a genotyping method. Although biochemical analysis suggested that most isolates were A. baumannii, genomic analysis revealed that the collection of Acinetobacter isolates comprised six different species, with non-baumannii Acinetobacter species representing the majority. All strains possessed an inherent ADC-type β-lactamase gene, whereas the distribution of OXA-type enzymes was limited to A. baumannii, A. pittii, and A. colistiniresistens. While MLST properly discriminated four A. baumannii strains as different clones, POT failed to distinguish three out of the four A. baumannii strains from each other, highlighting a potential pitfall that may be encountered when applying POT to non-epidemiological A. baumannii strains.

KEYWORDS:

Acinetobacter; PCR-based open reading frame typing; antimicrobial resistance; epidemiology; whole-genome sequencing

PMID:
30814458
DOI:
10.7883/yoken.JJID.2018.403
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