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J Transl Med. 2019 Feb 27;17(1):56. doi: 10.1186/s12967-019-1807-5.

Jaw osteosarcoma models in mice: first description.

Author information

1
Laboratoire des sarcomes osseux et remodelage des tissus calcifiés (Phy.OS), UMR 1238, Faculté de médecine, 1 rue Gaston Veil, 44035, Nantes Cedex, France. helios.bertin@chu-nantes.fr.
2
Service de chirurgie Maxillo-faciale et stomatologie, CHU de Nantes, 1 place Alexis Ricordeau, 44093, Nantes Cedex 1, France. helios.bertin@chu-nantes.fr.
3
Faculty of Population Health Sciences, UCL Institute of Child Health, 30 Guilford Street, London, England, WC1N 1EH, UK.
4
Laboratoire des sarcomes osseux et remodelage des tissus calcifiés (Phy.OS), UMR 1238, Faculté de médecine, 1 rue Gaston Veil, 44035, Nantes Cedex, France.
5
Service d'anatomie et cytologie pathologique, CHU de Nantes, 1 place Alexis Ricordeau, 44093, Nantes Cedex 1, France.
6
Service d'anatomie et cytologie pathologique, Institut Universitaire du Cancer Toulouse Oncopôle, 1 avenue Irène Joliot-Curie, 31059, Toulouse Cedex 9, France.
7
Centre de ressources biologiques - Cancer, Institut Universitaire du Cancer Toulouse Oncopôle, 1 avenue Irène Joliot-Curie, 31059, Toulouse Cedex 9, France.
8
Service de chirurgie Maxillo-faciale et stomatologie, CHU de Nantes, 1 place Alexis Ricordeau, 44093, Nantes Cedex 1, France.
9
Laboratoire Hétérogénéité Tumorale et Médecine de Précision, Institut de Cancérologie de l'Ouest, Boulevard Jacques Monod, 44805, Saint Herblain, France.
10
Service d'Histologie-Embryologie, Faculté de médecine de Nantes, 1 Rue Gaston Veil, 44035, Nantes, France.
11
Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire (LIOAD), Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042, Nantes, France.

Abstract

BACKGROUND:

Osteosarcoma (OS) is the most common cancer of bone. Jaw osteosarcoma (JOS) is rare and it differs from other OS in terms of the time of occurrence (two decades later) and better survival. The aim of our work was to develop and characterize specific mouse models of JOS.

METHODS:

Syngenic and xenogenic models of JOS were developed in mice using mouse (MOS-J) and human (HOS1544) osteosarcoma cell lines, respectively. An orthotopic patient-derived xenograft model (PDX) was also developed from a mandibular biopsy. These models were characterized at the histological and micro-CT imaging levels, as well as in terms of tumor growth and metastatic spread.

RESULTS:

Homogeneous tumor growth was observed in both the HOS1544 and the MOS-J JOS models by injection of 0.25 × 106 and 0.50 × 106 tumor cells, respectively, at perimandibular sites. Histological characterization of the tumors revealed features consistent with high grade conventional osteosarcoma, and the micro-CT analysis revealed both osteogenic and osteolytic lesions. Early metastasis was encountered at day 14 in the xenogenic model, while there were no metastatic lesions in the syngenic model and in the PDX models.

CONCLUSION:

We describe the first animal model of JOS and its potential use for therapeutic applications. This model needs to be compared with the usual long-bone osteosarcoma models to investigate potential differences in the bone microenvironment.

KEYWORDS:

Animal experimentation; Bone resorption; Environment; Mandible; Models; Sarcoma

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