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Nutrients. 2019 Feb 22;11(2). pii: E466. doi: 10.3390/nu11020466.

Interesterified Fats Induce Deleterious Effects on Adipose Tissue and Liver in LDLr-KO Mice.

Author information

1
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. mariasilviaferrari@yahoo.com.br.
2
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. milessafonso@gmail.com.
3
Laboratory of Nutritional Genomics-School of Applied Science, University of Campinas (UNICAMP), Limeira, São Paulo 13484-350, Brazil. dcintra@yahoo.com.
4
Emergency Care Research Unit Laboratory (LIM51), Faculty of Medical Sciences of the University of São Paulo, São Paulo 01246-903, Brazil. mkkoike17@gmail.com.
5
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. valeriasutti@gmail.com.
6
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. marinalgdemasi@gmail.com.
7
Laboratory of Molecular Biology (LIM22), Department of Pathology, Faculty of Medical Sciences of the University of São Paulo, São Paulo 01246-903, Brazil. cjlin@usp.br.
8
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. lismiem@yahoo.com.br.
9
Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences of the University of São Paulo, São Paulo 05508-000, Brazil. lagio@usp.br.
10
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. rebombo@comcast.net.
11
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. rmarcondesmachado@yahoo.com.br.
12
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. catanozi@usp.br.
13
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. enakonda@usp.br.
14
Laboratorio de Lipides (LIM10), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR 01246-903, Brazil. ana.lottenberg@hc.fm.usp.br.
15
Faculdade Israelita de Ciências da Saúde Albert Einstein, São Paulo, SP, BR 05521-200, Brazil. ana.lottenberg@hc.fm.usp.br.

Abstract

Interesterified fats are being widely used by the food industry in an attempt to replace trans fatty acids. The effect of interesterified fats containing palmitic or stearic acids on lipid metabolism and inflammatory signaling pathways in adipose and hepatic tissues was evaluated. Male LDLr-KO mice were fed a high-fat diet containing polyunsaturated (PUFA), palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR), or stearic interesterified (STEAR INTER) fats for 16 weeks. The expression of genes and protein levels involved in lipid metabolism and inflammatory processes in liver and white adipose tissue was determined by quantitative RT-PCR and by Western blot, respectively. The infiltration of inflammatory cells in hepatic and adipose tissues was determined by eosin and hematoxylin, while liver collagen content was determined by Sirius Red staining. Both interesterified fats increased liver collagen content and JNK phosphorylation. Additionally, the STEAR INTER group developed nonalcoholic steatohepatitis (NASH) associated with higher neutrophil infiltration. PALM INTER induced adipose tissue expansion and enlargement of adipocytes. Furthermore, PALM INTER triggered increased IKK phosphorylation and TNFα protein content, conditions associated with the upstream activation of the NFkB signaling pathway. STEAR INTER induced NASH, while PALM INTER triggered hepatic fibrosis and adipocyte hypertrophy with inflammatory response in LDLr-KO mice.

KEYWORDS:

NASH; inflammation; interesterified fat; lipids metabolism; obesity; saturated fatty acid

PMID:
30813339
PMCID:
PMC6412707
DOI:
10.3390/nu11020466
[Indexed for MEDLINE]
Free PMC Article

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