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Cell Rep. 2019 Feb 26;26(9):2307-2315.e5. doi: 10.1016/j.celrep.2019.01.104.

Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner.

Author information

1
Institute for Research in Biomedicine (IRB), Università della Svizzera italiana, Via Vincenzo Vela 6, 6500 Bellinzona, Switzerland; Graduate School of Cellular and Molecular Sciences, Faculty of Medicine, University of Bern, 3012 Bern, Switzerland.
2
Institute for Research in Biomedicine (IRB), Università della Svizzera italiana, Via Vincenzo Vela 6, 6500 Bellinzona, Switzerland.
3
Institute for Research in Biomedicine (IRB), Università della Svizzera italiana, Via Vincenzo Vela 6, 6500 Bellinzona, Switzerland; Institute of Computational Science (ICS), Università della Svizzera italiana, Via Giuseppe Buffi 13, 6900 Lugano, Switzerland.
4
Institute of Oncology Research (IOR), Via Vincenzo Vela 6, 6500 Bellinzona, Switzerland.
5
Institute of Oncology Research (IOR), Via Vincenzo Vela 6, 6500 Bellinzona, Switzerland; Laboratory of Molecular Biology, Instituto di Ricerche Farmacologiche Mario Negri IRCCS, via Giuseppe La Masa 19, 20156 Milano, Italy.
6
Institute for Research in Biomedicine (IRB), Università della Svizzera italiana, Via Vincenzo Vela 6, 6500 Bellinzona, Switzerland; Institute for Microbiology, ETH Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zurich, Switzerland.
7
Theodor Kocher Institute (TKI), University of Bern, Freiestrasse 1, 3000 Bern, Switzerland.
8
Institute for Research in Biomedicine (IRB), Università della Svizzera italiana, Via Vincenzo Vela 6, 6500 Bellinzona, Switzerland. Electronic address: santiago.gonzalez@irb.usi.ch.

Abstract

The role of natural killer (NK) cells in the immune response against vaccines is not fully understood. Here, we examine the function of infiltrated NK cells in the initiation of the inflammatory response triggered by inactivated influenza virus vaccine in the draining lymph node (LN). We observed that, following vaccination, NK cells are recruited to the interfollicular and medullary areas of the LN and become activated by type I interferons (IFNs) produced by LN macrophages. The activation of NK cells leads to their early production of IFNγ, which in turn regulates the recruitment of IL-6+ CD11b+ dendritic cells. Finally, we demonstrate that the interleukin-6 (IL-6)-mediated inflammation is important for the development of an effective humoral response against influenza virus in the draining LN.

KEYWORDS:

IFNγ; IL-6; dendritic cell; influenza vaccine; natural killer cell; type I IFN

PMID:
30811982
DOI:
10.1016/j.celrep.2019.01.104
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