Format

Send to

Choose Destination
Stem Cell Res. 2019 Feb 20;36:101411. doi: 10.1016/j.scr.2019.101411. [Epub ahead of print]

Generation of induced pluripotent stem cell line, ZJUCHi002-A, from Charcot-Marie-Tooth disease type 2A (CMT2A) patient with a mutation of c.752C>T in MFN2.

Author information

1
The Children's Hospital Affiliated & Key Laboratory of Diagnosis and Treatment of Neonatal Diseases of Zhejiang Province, School of Medicine, Zhejiang University, Hangzhou 310052, China; Institute of Translational Medicine, School of Medicine, Zhejiang University, Hangzhou 310020, China.
2
The Otolaryngological Department, The Children's Hospital, School of Medicine, Zhejiang University, China.
3
Zhejiang Center for Disease Control and Prevention, China.
4
The Pediatric Rehabilitation Department, The Children's Hospital, School of Medicine, Zhejiang University, Hangzhou 310052, China.
5
Experimental Animal Center, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
6
Department of neurosurgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
7
The Pediatric Rehabilitation Department, The Children's Hospital, School of Medicine, Zhejiang University, Hangzhou 310052, China. Electronic address: 6199005@zju.edu.cn.
8
The Children's Hospital Affiliated & Key Laboratory of Diagnosis and Treatment of Neonatal Diseases of Zhejiang Province, School of Medicine, Zhejiang University, Hangzhou 310052, China; Institute of Translational Medicine, School of Medicine, Zhejiang University, Hangzhou 310020, China; Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, School of Medicine, Zhejiang University, Hangzhou 310058, China. Electronic address: chenwei001@zju.edu.cn.

Abstract

The human induced pluripotent stem cell (iPSC) line ZJUCHi002-A was established from renal epithelial cells present in urine (urinary cells) collected from an 8-year-old Charcot-Marie-Tooth disease type 2A (CMT2A) patient carrying point mutation in MFN2 (c.752C > T). Urinary cells were reprogrammed by retrovirus vectors containing reprogramming factors: OCT4, SOX2, KLF4 and c-MYC. The pluripotency, capacity of differentiation into 3 germ layers, silence of reprogramming factors and normal karyotype were all confirmed in this study.

PMID:
30807887
DOI:
10.1016/j.scr.2019.101411
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center