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J Parasitol. 2019 Feb;105(1):133-134.

Identification of Arthropods By Polymerase Chain Reaction As Probes For Infectious Disease Studies In Experimental Coprolites.

Author information

1
1   Laboratory of Parasite Molecular Biology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24210-130 Brazil.
2
2   Laboratory of Hantaviruses and Rickettsiosis, FIOCRUZ, Rio de Janeiro 21040-900 Brazil.
3
3   Laboratory of Paleoparasitology, FIOCRUZ, Rio de Janeiro 21040-900 Brazil.
4
4   Laboratory of Helminthology Romero Lascassa Porto, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-900 Brazil.

Abstract

The study of arthropods is still scarce in paleoparasitology, especially their molecular identification. In this experimental study, we amplify DNA using a polymerase chain reaction from 2 ectoparasite species, Rhipicephalus sanguineus and Pediculus humanus capitis, in experimentally desiccated feces. This study shows perspectives for the study of the identification of arthropods in coprolites when taxonomic identification is not possible.

KEYWORDS:

Paleoparasitology; Ectoparasites; PCR; Vector-Borne Diseases; Vectors

PMID:
30807730

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